Mispriming library with Primer 3
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9.6 years ago
sfcarroll ▴ 80

In the Primer 3 manual, it details where fasta format mispriming libraries can be found on the internet, eg:

The documentation for mispriming libraries can be found here

Is the correct way to use these libraries like this:

  1. Download the mispriming libraries, and save them as .fa, eg: human.fa
  2. When running Primer 3 from the command line, pass the appropriate mispriming library path.

e.g.:

PRIMER_MISPRIMING_LIBRARY='/home/primer3/mispriming/human.fa'

Is this correct?

PCR • 4.0k views
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