Question: How to deal with cross-hybridizing Affymetrix (or other) probe sets?
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gravatar for scottwilliamrobinson
6.1 years ago by
United Kingdom
scottwilliamrobinson130 wrote:

Some probe sets on microarrays cross-hybridise (hybridise to several target genes). If all possible targets were included this could skew the analysis in the favour of the cross-hybridising targets, however if only one is selected (at random?) or if the probe sets are left out entirely you might miss something important.

Is there any general concensus on how to deal with this problem?

ADD COMMENTlink modified 6.1 years ago • written 6.1 years ago by scottwilliamrobinson130
1
gravatar for Ctanes
6.1 years ago by
Ctanes70
United States
Ctanes70 wrote:

I would recommend using custom cdf files. They redefine the probe sets so that it's one to one mapping to the genes. I worked with Affymetrix arrays and use the cdf files here: http://brainarray.mbni.med.umich.edu/brainarray/default.asp They keep a very good update of their cdf files as new mRNA information is released.

ADD COMMENTlink written 6.1 years ago by Ctanes70

I had read about these recently actually, I think I will try them out. I hadn't noticed before that they supply both gene-centric and transcript-centric CDFs, that's nice.

ADD REPLYlink written 6.1 years ago by scottwilliamrobinson130
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gravatar for scottwilliamrobinson
6.1 years ago by
United Kingdom
scottwilliamrobinson130 wrote:

I asked the maintainer of the Cytoscape GO term enrichment plug-in ClueGO how they handle cross-hybridising Affy IDs (as you can input them directly): "For the moment, we allow several affy spots for one entrez id, but only one entrez id for one affy spot. In this last case, we display the gene with the lowest entrez id (oldest annotation)."

ADD COMMENTlink modified 6.0 years ago • written 6.1 years ago by scottwilliamrobinson130
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