I am trying to parse Humicola genome "embl" format, and extract all its gene id. I quite new in this stuff, so may need some help in termology as well ! Seems in the file, there is more than one section for features (meaning it could be more than 1 genome).how do you call these part in flatfile ?!
Anyway, so for this purpose, I wrote a small python script and used BioPython,
embl = dict() handle = open('MyGenome.embl', "rU") for cnt, record in enumerate(SeqIO.parse(handle, "embl")) : print len(record.features) print cnt handle.close()
but I am getting the following error in 10th record.features.(could be because its corrupted or there is bug withing my biopython (version 1.58) or may be you know an easier parse!)
here is the error
File "/usr/lib/pymodules/python2.6/Bio/GenBank/Scanner.py", line 432, in parse_records record = self.parse(handle, do_features) File "/usr/lib/pymodules/python2.6/Bio/GenBank/Scanner.py", line 415, in parse if self.feed(handle, consumer, do_features): File "/usr/lib/pymodules/python2.6/Bio/GenBank/Scanner.py", line 387, in feed self._feed_feature_table(consumer, self.parse_features(skip=False)) File "/usr/lib/pymodules/python2.6/Bio/GenBank/Scanner.py", line 184, in parse_features features.append(self.parse_feature(feature_key, feature_lines)) File "/usr/lib/pymodules/python2.6/Bio/GenBank/Scanner.py", line 281, in parse_feature assert len(qualifiers) > 0
Where I track genes that I am tracking, the one after last one in embl file is like
FT exon complement(494..1420) FT /note="exon_id=CADAORAE00020594"
and after that, another section (genome) start,which is look like this
ID XCFFLD351 Unannotated; unspecified; UNC; 494655 BP. XX AC Z2573200; XX DT 01-OCT-2010 (Rel. 01, Created) DT 01-OCT-2010 (Rel. 01, Last updated, Version 1) XX DE Humicola xisolens XX KW genomic. XX OS Humicola xisolens O45MC-2 OC Eukaryota; Fungi; OC . XX RN  RP 1-494655 RA MXKA; RT Humicola xisolens RL email@example.com; XX DR seq; PC; O45PC. CC length=494655 XX FH Key Location/Qualifiers FH FT CDS complement(join(178..370,439..529,588..678,1009..1060, FT 1584..1773,1831..1948,2838..2879)) FT /protein_id="PE04150001210" FT /db_xref="PEDANT:PE04150001210"
Also, I am wondering how can I extract whole the part that causing error, and paste here ?!! I know its 10th record.feature! here is the file