Hey All I have data frame with 5 Samples A,B,C,D,E.
A is parent (reference)sample and rest of samples are from patients. each row represents a miRNA and value against that row in each column represents Back ground subtraction values of that miRNA in each sample. I want to perform ANOVA test in R. I am bit confused how I should perform either with Parent and one patient sample (A&B) A&C and so on). Secondly most of the ANOVA tests which I saw on google and youtube they have for example one column with data second column with different groups for the value for example
Weight Loss Diet 1.2 A 22.3 A 5.4 C 33.5 B etc A B C D hsa-miR-199a-3p, hsa-miR-199b-3p NA 13.13892 5.533703 25.67405 hsa-miR-365a-3p, hsa-miR-365b-3p 15.70536 52.86558 18.467540 223.51424 hsa-miR-3689a-5p, hsa-miR-3689b-5p NA 21.41597 5.964772 NA hsa-miR-3689b-3p, hsa-miR-3689c 9.58696 44.56490 10.102051 13.26785 hsa-miR-4520a-5p, hsa-miR-4520b-5p 18.06865 28.06991 NA NA hsa-miR-516b-3p, hsa-miR-516a-3p NA 10.77471 8.039662 NA E hsa-miR-199a-3p, hsa-miR-199b-3p NA hsa-miR-365a-3p, hsa-miR-365b-3p 31.93503 hsa-miR-3689a-5p, hsa-miR-3689b-5p 24.26073 hsa-miR-3689b-3p, hsa-miR-3689c NA hsa-miR-4520a-5p, hsa-miR-4520b-5p NA hsa-miR-516b-3p, hsa-miR-516a-3p NA
How I should do for my data
Thanks in Advance