I have modeled a membrane protein which has 3 subunits- alpha, beta, gamma. Now, as the native structure of the protein is pentamer, I would like to create the pentamer having 2 alpha, 2 beta and 1 gamma subunit (all modeled pdb files) oriented alternatively. Can anybody suggest some tools for creating the multimeric protein (monomer association)? How do I know if the subunit interfaces are correctly predicted in the generated heteromer?
Thanks in advanced.