Question: PCR annealing analysis
0
gravatar for Bioinf/Biotech
5.5 years ago by
Bioinf/Biotech0 wrote:

I was wondering if theoretically we have a long DNA template that has to be amplified and, for instance, add an incorrect primer that has the same directionality as the template (5'->3'), but is complementary to the strand, will it anneal? I understand that it won't be amplified, but purely from biochemical/ biophysical standpoint annealing should occur, since the complementarity is primarily defined by the base paring?

ADD COMMENTlink written 5.5 years ago by Bioinf/Biotech0

Hi,

I'm not sure the question pertains to bioinformatics. We're a community of less wet bench specialists, more biological data scientists.

ADD REPLYlink modified 5.5 years ago • written 5.5 years ago by RamRS27k
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