I was wondering if theoretically we have a long DNA template that has to be amplified and, for instance, add an incorrect primer that has the same directionality as the template (5'->3'), but is complementary to the strand, will it anneal? I understand that it won't be amplified, but purely from biochemical/ biophysical standpoint annealing should occur, since the complementarity is primarily defined by the base paring?
Question: PCR annealing analysis
5.5 years ago by
Bioinf/Biotech • 0
Bioinf/Biotech • 0 wrote:
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