Question: (Closed) Best control in RNAseq for homozygous zebrafish mutant in transgenic background
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gravatar for mgoi
4.3 years ago by
mgoi0
Canada
mgoi0 wrote:

What is the best control in RNAseq analysis for an homozygous mutant in transgenic background? (for zebrafish) 

Should it be an heterozygous mutant in the same transgenic background? Could it be a regular transgenic or also a wild-type non transgenic? 

Thanks!

rna-seq • 868 views
ADD COMMENTlink written 4.3 years ago by mgoi0

This question cannot be sufficiently answered at this level of detail. What can be seen a suitable experimental design depends on many factors and of course the biological question. 

ADD REPLYlink written 4.3 years ago by Michael Dondrup45k

Hello mgoi!

We believe that this post does not fit the main topic of this site.

This question on experiment design cannot be answered because lack of well defined hypothesis to test.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 4.3 years ago by Michael Dondrup45k
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