Bioinformatics noob here,
I just downloaded the blast command line applications and I would like to make a database of a transcriptome that I have acquired. It is labeled as firefly.fas and I have put the file into a folder on my desktop in the path /Desktop/Science/ncbi/.
Ive been typing this into the command line:
-bash: Desktop/Science/ncbi/: is a directory
makeblastdb -in firefly.fas -dbtype nucl -parse_seqids -out FF
Building a new DB, current time: 12/18/2014 15:23:12
New DB name: FF
New DB title: firefly.fas
Sequence type: Nucleotide
Keep Linkouts: T
Keep MBits: T
Maximum file size: 1000000000B
BLAST options error: File firefly.fas does not exist
Everytime I always get that the files does not exist. I am sure that it is a fasta file, and that it is in the right folder. I even tried sample fasta sequences that I downloaded and got the same error. It is probably a simple problem to solve, but I cannot seem to get past it.