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12.6 years ago
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I am a newbie in the field of Chip-Seq and would like to gain some knowledge from the experts,I am planning to do Chip seq experiments for the Histone marks,H3k4 mono methyl and K27 Acetyl.
I am a bit confused about the validation,using PCR or qPCR.
What should be my positive and negative controls.Which all gene specific primers can I use,I am not able to understand,due to the confusion.I want to run a pre check,before sequencing,about the modification specificity.Whether the DNA pooled is related to the Histone marks,and they can be distinguished using PCR.
Suggestions and comments are welcome.
