build hg38 in CEAS
1
0
Entering edit mode
9.3 years ago
chxu02 ▴ 10

Hi guys,

I tried to build hg38 in CEAS using command:

$ build_genomeBG -d hg38 -g refGene -w hg38.wig -o hg38.refGene

I made this hg38.wig and it looked like:

track type=wiggle_0
fixedStep chrom=chr1 start=1 step=248956422
1
fixedStep chrom=chr2 start=1 step=242193529
1
......

It seemed all good, and the annotation table was successfully created.

But when I run ceas:

$ ceas -g hg38.refGene -b Bio.bedgraph

A graph was generated, with no information about the genome (all showing bar value of 0), while the intervals in my bed seemed to be allocated into each category in a reasonable way.

What's going on?

ChIP-Seq gene genome • 3.2k views
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Entering edit mode

Hi,

I am experiencing the same problem for mm10. Did you find a solution for this?

Thanks

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Entering edit mode
9.1 years ago
ctk2 ▴ 10

You can simply add the flag --bg when you run ceas. Than it will properly show the reference genome data.

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