Forum:Pipeline for a meta-assembly of De-novo Transcriptome

Question

[giorgiocasaburi]

Hi all,

I'm about to analyze a De Novo transcriptome assembly and I have more than 500GB from different runs (both 454 and Illumina). I was thinking to analyze the 454 with Mira or Newbler and the Illumina with Trinity. Further, merging all the obtained contings using CAP3. What do you think? Does anyone have any experience with co-assembly?

Thanks!