Hi all, I would like to know how can I can calculate sequencing depth for amplicon sequencing.
I am conducting a microbial survey sequencing 16S rRNA´s V3-V4 region which yields an amplicon of ~450 pb. So for each sample I have a mixture of different amplicon size (around ~450pb) for each bacteria´s 16S that got amplified with the primers I used.
I would like to use the The Lander / Waterman equation C=NL/G, but since I am not sequencing a whole genome but a set of unknown quantity of bacteria´s 16S, I don´t know what number to put in. I am actually not even sure if it´s possible to calculate it, at least with that formula..
Please help, Cheers