I have set of mature miRNA sequences (18-36 bp range)[genome sequence is not available]. For these mature mirna sequences i want to find exact precursor sequences. I aligned mature sequence to genome and took upstream and downstream 50 bp including mature mirna and did the rna folding but i am getting structure for full. my questions are
1) How do i predict exact precursor sequence
2) To identify miRNA* for existing mature rna. Should i just reverse complement the mature mirna?
any suggestions are appreciated. Thanks