I have set of mature miRNA sequences (18-36 bp range) [genome sequence is not available]. For these mature mirna sequences I want to find exact precursor sequences. I aligned mature sequence to genome and took upstream and downstream 50 bp including mature mirna and did the rna folding but I am getting structure for full. my questions are
- How do I predict exact precursor sequence
- To identify miRNA* for existing mature rna. Should I just reverse complement the mature mirna?
Any suggestions are appreciated. Thanks