Question: Two-Color Array with Sample in One Color and Reference in Another
gravatar for Nifaste
5.6 years ago by
Nifaste20 wrote:

Dear all,

I'm analyzing microarray in which each experimental sample (labeled with Cy5) is hybridized against a common reference sample (labeled with Cy3). Samples were obtained from wild-type and differents mutants. For each sample, i have 6 biological repicates. So finally i've 36 experiments.

In a first time, i've to determine the mRNA expression level in wild-type condition (in order to have a liste of mRNA that are expressed).

I'm little confused, i don't know how to proceed.

The data are stored in a RGList where RGList$R are raw data value for wild-type and RGList$G are raw data for the reference.

In a first step I did :


And i wanted to start with the A-value .... What do you think about that ?



microarray bioconductor R • 2.1k views
ADD COMMENTlink modified 5.5 years ago by Biostar ♦♦ 20 • written 5.6 years ago by Nifaste20

If you mention what package you use and show the structure of the data, things might be a lot clearer for people wanting to give an answer.

ADD REPLYlink written 5.4 years ago by olle.nordesjo0
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