I'm analyzing microarray in which each experimental sample (labeled with Cy5) is hybridized against a common reference sample (labeled with Cy3). Samples were obtained from wild-type and different mutants. For each sample, I have 6 biological replicates. So finally I've 36 experiments.
In a first time, I've to determine the mRNA expression level in wild-type condition (in order to have a liste of mRNA that are expressed).
I'm little confused, I don't know how to proceed.
The data are stored in a RGList where
RGList$R are raw data value for wild-type and
RGList$G are raw data for the reference.
In a first step I did:
And I wanted to start with the A-value. What do you think about that?