Metagenomics Pipelines
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12.5 years ago
David • 0

Want to start doing shotgun metagenomics with mixed microbial community samples. Will use 454 as sequencing platform with long reads (approx 750 bases). What is the best analysis pipeline to use to compare across samples; microbial diversity at genus, species level and relative gene contribution so I can infer functional differences?

metagenomics next-gen sequencing • 3.3k views
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Are you sequencing whole genomic DNA, or just 16s rRNA ?

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