Question: Should I retain ORFs predicted on minus strand of transcripts which assembled from strand-specific RNA-seq ?
gravatar for sckinta
5.3 years ago by
United States
sckinta580 wrote:


I assembled a transcriptome using Trinity with  setting of --SS_lib_type R, then I continued to predict ORFs using TransDecoder. The final result gives me 236711 ORFs with 202113 on + strand of transcripts. This is a good sign to show strand-specific setting worked. but I am not sure whether I should retain those ~ 20000 ORFs predicted on - strand.

I will further do DE analysis and SNP calling. Will retaining those minus-strand ORF affect my following analysis?

Thank you,


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