I have two questions about the somatic mutation.
1. I researched the somatic mutation(indels) in cancer sample. And now I want to detect the somatic indels in blood sample(autism). However it is hard to find the somatic indels because it is blood sample.
In cancer sample there are two types of samples like normal sample and tumor sample but in blood sample for autism there is only one type of sample. (Blood sample is undistinguished)
So how can I get the somatic indels in blood sample?
2. Also I wonder the detailed principle for detecting the somatic mutation in VarScan2 and Scalpel.