Building A Dimer From No Template
Entering edit mode
12.4 years ago
Harpalss ▴ 310

I have built a homology model for a monomer of a DNA protein. This protein is also known to dimerise. The dimerisation site had been identified.

What would be the easiest way to building a Dimer from the monomer I built taking the dimerisation site into account?

modeling • 3.4k views
Entering edit mode
12.4 years ago
Neilfws 49k

I'm reasonably sure that you cannot build a dimer by homology modeling unless you have a template (known structure) of a dimer. The dimerisation site alone is insufficient information.

See for example replies on the Modeller mailing list - reply 1, reply 2 and this information from SwissModel (scroll down to "Example: Modelling a dimeric protein").

If your protein is small and you have the resources (and know-how), molecular dynamics simulation might be an option. See for example How the hell do I model this thing by Bosco.


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