Instead of using limma on cel files downloaded from GEO, I now have gene expression data for differential expression of genes under various condition (8 replicates) in txt file in following format:
Gene Name Condition1 Condition 2 ..... Condition 8
Gene A 11.235 10.4657 ...... 9.34567
Gene B 15.324 11.334 ...... 16.3245
I was following this tutorial but as it uses cel files as input so I was encountering problem in fitting the linear model on my expression dataset.
Can someone guide me how I can further proceed with limma if I have data in such format?
Thanks for your suggestions.