Question: (Closed) I want to check if a disease is heritable and what is the chance that the offspring will have the same disease in near future .
0
gravatar for Being Bioinformatician
4.4 years ago by
India
Being Bioinformatician170 wrote:

Respected Members,

 I want to check if a disease is heritable and what is the chance that the offspring will have the same disease in near future .

Which procedure should i follow (RT-PCR or PCR-RFLP or any other procedure )... I need some protocol

Thanks for Help  in advance !!!

gene • 802 views
ADD COMMENTlink modified 4.4 years ago • written 4.4 years ago by Being Bioinformatician170

Thanks all for your answer .

ADD REPLYlink written 4.4 years ago by Being Bioinformatician170

Hello Being Bioinformatician!

We believe that this post does not fit the main topic of this site.

Not relevant enough to bioinformatics

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 4.4 years ago by Michael Dondrup46k
3
gravatar for Devon Ryan
4.4 years ago by
Devon Ryan92k
Freiburg, Germany
Devon Ryan92k wrote:
  1. This isn't really a bioinformatics question, it's just about which molecular biology method to use.
  2. The answer depends on the nature of the disease. If it's due to a CNV, then RT-PCR would be useful. If it's due to a SNP or InDel then PCR-RFLP might work, though I'd personally just do sequencing of the whole gene.

This all assumes a simple Mendelian disorder, of course.

ADD COMMENTlink written 4.4 years ago by Devon Ryan92k
3

If the OP is asking about whether or not it is even heritable then you are starting even earlier than that in the process. Whether it is even a genetic disease. If for some reason the OP thinks it is genetic then the standard would be to see if it is or is similar to other known Mendelian disease. You can start by testing those genes known to cause the same or similar diseases, or go straight to something like Exome sequencing.

ADD REPLYlink written 4.4 years ago by Dan Gaston7.1k
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gravatar for dariober
4.4 years ago by
dariober10k
WCIP | Glasgow | UK
dariober10k wrote:

I want to check if a disease is heritable

As Dan Gaston hints to, you don't need a molecular biology technique for this. Depending on your study system you can e.g. regress the phenotype of the offspring on the average phenotype of the parents. The slope of the regression line is the heritability. Molecular biology techniques then allow you to map the gene(s) underlying the phenotype.

ADD COMMENTlink written 4.4 years ago by dariober10k
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