Following my post (RNA-seq triple replicates), I have a further question about evaluation of RNA-seq replicates. Previously my colleagues faced a situation, in which a reviewer in a journal requested RNA-seq replicates. I think this is probably true for many of you. Therefore, RNA-seq replicates are definately necessary.
I want to ask you how to evaluate RNA-seq quality if having three replicates (I think it is easier for two replicates, as scatter plot is enough). Are there any tools avaliable to statistically assess RNA-seq quality? THANK YOU very much!