I've been trying to run MetaVelvet assembly of an environmental DNA sample (short, paired end, fastq, illumina reads) on a supercomputer cluster, and have been unable to generate output. I am using Velvet 1.2.10 and MetaVelvet -1.1.01. The program will run to completion (in a fraction of a second) on very small files (containing 20,000 sequences each) for both single and paired end reads, and produce a functional meta-velvet contigs file. Vevleth and Velvetg both run successfully on files containing 40,000 sequences each. However, when running MetaVelvetg on these results, the program will run until reaching the wall timer.
I am using the following sequence of commands:
- mpirun -np $PBS_NP ~/bin/velvet-master/velveth $fol 31 -fastq -shortPaired $sequence1 $sequence2
- mpirun -np $PBS_NP ~/bin/velvet-master/velvetg $fol -read_trkg yes -scaffolding no
- mpirun -np $PBS_NP ~/MetaVelvet-1.1.01/meta-velvetg $fol -scaffolding no
$sequence2 point to their respective ends of each sequence, and
$fol points to the output directory. I have used a different output directory with each run. I typically run the process with 6 nodes, each with 8 2.66 GHz processors and access to some allocation of 2.62 TB shared memory. I have tried up to 24 of those nodes, and MetaVelvet has never run to completion, so it seems like it's not a matter of processing power.
When I've killed the process, the error file contained the following:
MPI: could not run executable (case #3) MPI: No details available, no log files found /opt/torque/4.2.9/spool/mom_priv/jobs/77068.hokieone.SC: line 34: 369997 Killed mpirun -np $PBS_NP /home/slvt16/bin/velvet-master/velveth $fol 31 -fastq $sequence1 MPI: could not run executable (case #3) MPI: No details available, no log files found /opt/torque/4.2.9/spool/mom_priv/jobs/77068.hokieone.SC: line 35: 374736 Killed mpirun -np $PBS_NP /home/slvt16/bin/velvet-master/velvetg $fol -read_trkg yes -scaffolding no
The output log shows that the program is hanging on the ------Scafolding------ step, even when run with the '-scaffolding no' flag.
I would like to run MetaVelvet on paired end files of ~400,000 reads each, eventually. I'm running out of ideas for troubleshooting, so any help would be appreciated!
EDIT: Also on: http://seqanswers.com/forums/showthread.php?t=61120