NCBI BLAST Search Results order by ASC
Entering edit mode
7.6 years ago
clear.choi ▴ 30

Hello I have blast search results as below,

BLASTN 2.2.29+

Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.

Database: /shared/MiSeq/ABC_Exon23.fasta
           7,546 sequences; 4,346,452 total letters

Query= BarcodeS14--S14_Cluster0_Phase0_NumReads236

                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  C_08_94                                                               510   7e-145
  C_08_90                                                               510   7e-145
  C_08_75                                                               510   7e-145
  C_08_74                                                               510   7e-145
  C_08_73                                                               510   7e-145
  C_08_69                                                               510   7e-145
  C_08_68                                                               510   7e-145
  C_08_67                                                               510   7e-145
  C_08_55N                                                              510   7e-145
  C_08_53                                                               510   7e-145
  C_08_52N                                                              510   7e-145
  C_08_45                                                               510   7e-145
  C_08_37                                                               510   7e-145
  C_08_28                                                               510   7e-145
  C_08_25                                                               510   7e-145
  C_08_18                                                               510   7e-145
  C_08_17                                                               510   7e-145
  C_08_05                                                               510   7e-145
  C_08_02_08                                                            510   7e-145

<allele lists>

Lambda      K        H
    1.33    0.621     1.12

Lambda      K        H
    1.28    0.460    0.850

Effective search space used: 4101954802

  Database: /shared/MiSeq/ABC_Exon23.fasta
    Posted date:  Jul 22, 2014  5:00 PM
  Number of letters in database: 4,346,452
  Number of sequences in database:  7,546

I can get only top matching sequence so I can give option like below,

blastn -db .DB/ABC_Exon23.fasta.db -query ./test.fasta -out ./test.out -num_descriptions 1 -num_alignments 1-word_size 10

But then results will be

C_08_94                                                               510   7e-145

Actually I want to get below

C_08_02_08                                                            510   7e-145

So I want to order by ASC of results and get an top one.

PS. Top one has to be top score, order priory 1. Score 2. Name.

Anyone has good idea?


NCBI blast • 1.8k views

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