Question: NCBI BLAST Search Results order by ASC
gravatar for clear.choi
5.1 years ago by
United States
clear.choi30 wrote:

Hello I have blast search results as below,


BLASTN 2.2.29+

Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb
Miller (2000), "A greedy algorithm for aligning DNA sequences", J
Comput Biol 2000; 7(1-2):203-14.

Database: /shared/MiSeq/ABC_Exon23.fasta
           7,546 sequences; 4,346,452 total letters

Query= BarcodeS14--S14_Cluster0_Phase0_NumReads236

                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  C_08_94                                                               510   7e-145
  C_08_90                                                               510   7e-145
  C_08_75                                                               510   7e-145
  C_08_74                                                               510   7e-145
  C_08_73                                                               510   7e-145
  C_08_69                                                               510   7e-145
  C_08_68                                                               510   7e-145
  C_08_67                                                               510   7e-145
  C_08_55N                                                              510   7e-145
  C_08_53                                                               510   7e-145
  C_08_52N                                                              510   7e-145
  C_08_45                                                               510   7e-145
  C_08_37                                                               510   7e-145
  C_08_28                                                               510   7e-145
  C_08_25                                                               510   7e-145
  C_08_18                                                               510   7e-145
  C_08_17                                                               510   7e-145
  C_08_05                                                               510   7e-145
  C_08_02_08                                                            510   7e-145

<allele lists>

Lambda      K        H
    1.33    0.621     1.12

Lambda      K        H
    1.28    0.460    0.850

Effective search space used: 4101954802

  Database: /shared/MiSeq/ABC_Exon23.fasta
    Posted date:  Jul 22, 2014  5:00 PM
  Number of letters in database: 4,346,452
  Number of sequences in database:  7,546


I can get only top matching sequence so I can give option like below,

blastn -db .DB/ABC_Exon23.fasta.db -query ./test.fasta -out ./test.out -num_descriptions 1 -num_alignments 1-word_size 10

But then results will be

C_08_94                                                               510   7e-145

Actually I want to get below

C_08_02_08                                                            510   7e-145


So I want to order by ASC of results and get an top one.

PS. Top one has to be top score , order priory 1. Score 2. Name.

Anyone has good idea?


asc blast ncbi autotyping • 1.3k views
ADD COMMENTlink modified 5.0 years ago by Biostar ♦♦ 20 • written 5.1 years ago by clear.choi30
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