I am trying to calculate the bisulfite conversion rates in WGBS/RRBS experiments. Bismark generates a nice report like this:
Final Cytosine Methylation Report ================================= Total number of C's analysed: 86084529 Total methylated C's in CpG context: 9308262 Total methylated C's in CHG context: 84996 Total methylated C's in CHH context: 131989 Total C to T conversions in CpG context: 5029566 Total C to T conversions in CHG context: 19274244 Total C to T conversions in CHH context: 52255472 C methylated in CpG context: 64.9% C methylated in CHG context: 0.4% C methylated in CHH context: 0.3%
This seems to be the numbers that I need to use, but I am not sure how I get the bisulfite conversion rate from them. Is it possible from a single sample or do I need to have both bisulfite treated and untreated samples?