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9.7 years ago
shawncho
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Before working with Next-Gen machines or datasets, it's vital to understand how the technology works. Here's the first part of a guide to Next-Generation Sequencing techniques that covers emulsion PCR, sequencing by ligation, polony sequencing, 454 Roche Pyrosequencing, and Ion Torrent.
http://binf.snipcademy.com/lessons/ngs-techniques
SOLiD Platform and Illumina SBS will come in the next series.
Also, for basic DNA sequencing techniques (maxam gilbert, sanger sequencing, primer walking, reversible chain terminators, shotgun sequencing) check out: http://binf.snipcademy.com/lessons/dna-sequencing-techniques