How to get a list of differentialy expressed genes with data from Illumina array?
0
1
Entering edit mode
8.7 years ago
kanacska ▴ 10

Hi,

I want to do a list for differentially expressed genes with txt data from this experiment: GSE38376

I downloaded the txt file read it in R and normalized it:

> idata <- read.ilmn("GSE38376_non-normalized.txt",probeid = "PROBE_ID",expr="SKBR")
> data_nobg <- backgroundCorrect(idata,method="normexp")
> data_norm <- normalizeBetweenArrays(data_nobg,method="quantile")

data_norm is in Elist class

Now I would like to select the differentially expressed genes with creating a model.matrix and then do the lmFit but do I have to do the step ExpressionSetillumina? if I do how do I do do it , with the class Elist? If id ont how do I continue??

Thanks,
Anna

microarray illumina limma R • 1.7k views
ADD COMMENT

Login before adding your answer.

Traffic: 1790 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6