I am trying to construct a gene model of new species using augustus3.1.
I have some RNAseq data, so I utilized it for 'intron hints'.
Augustus tutorial says FilterBam program can be used for more accurate gene prediction (http://bioinf.uni-greifswald.de/bioinf/wiki/pmwiki.php?n=IncorporatingRNAseq.Tophat), so I decided to use it.
However, everytime I run filterBam, it always ended up with message like this:
processed line 74100terminate called after throwing an instance of 'std::bad_alloc' what(): std::bad_alloc"
Despite of this message filtered BAM file was generated but I do not know this BAM file is reliable or not.
Where I went wrong?