looking for a server for processing data from Nextseq 500
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7.3 years ago
johnsunx1 • 0

Hi everyone,

We just buy a nextseq 500 and plan to buy a new server for processing the sequencing data, including exome-capture, RNA-Seq, ChIP-Seq, etc. Our budget for server is around $100K, and we are hunting for the server brand, configuration, etc. Do you have experience with nextseq 500? Any suggestion for the server is highly appreciated!

Thank you!

illumina nextseq500 • 1.6k views
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At a minimum, I would recommend 64 core Intel Xeon processors, 512GB ram and 60TB of hard disk space. This would be sufficient to handle 2-3 projects at a time (like alignments and variant calling/peak calling).

As the sequencing data keeps on adding up, you may need to keep the backup of sequencing runs, hence the hard disk should be bought accordingly.

Depending on the number of projects, you may need to increase the computational capacity. Your budget seems pretty good. You should be able to get a far better configured server.

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It would probably be far cheaper hardware-wise to have a cluster of lower-end nodes, say 4x128g 16-core.

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