Get ASCII-format PSSM from web PSIBLAST
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6.1 years ago
fvillanelo • 0

Hi everybody

I need to run a very large PSI-BLAST (using up to 10000 seqs and nr database) to extract PSSM but and I cannot use local PSI-BLAST because I ran out of RAM. So I used the web blast without problem but the PSSM is able to download only in ASN.1 format.

Reading here I have found that ASN.1 format is very hard to understand. Nevertheless I found I way to convert to ASCII, but I can retrieve only the 20 columns corresponding to the 20 Aa normalized scores per position in the PSSM. My problem is I need the information per position, and relative weight of gapless real matches to pseudocounts. These information is saved in the ASCII-PSSM of local blast (psiblast from ncbi-tools or blastpgp from blast) but is absent in ASN.1-PSSM from web blast.

So my questions are:

- is there a way to save ASCII-PSSM directly from web blast (this guide say it is possible, but seems to be an old document because it is not possible to follow the instructions there)

- is the information regarding information per position, and relative weight of gapless real matches to pseudocounts hidden in the ASN.1-PSSM from web blast

Thanks,

blast psiblast pssm • 3.6k views
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Hi, I'm not sure if you've solved your problem, but I've a similar question. How did you find a way to convert ASN.1 to ASCII

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Hi, please only post answers if you’re actually answering the question. I’ve moved your post now. If you have a question, it would be best to ask it in a new thread, not least because this one has been dead for 3.5 years.

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