Question: Per base sequence quality before and after truseq adapters removal
gravatar for cristianrohr768
3.3 years ago by
cristianrohr76830 wrote:

Hello all,

I have Illumina Truseq data from a custom design sequenced on a MiSeq. Before removal of adapters sequences, quality from position starting to 85bp (reads of 151 bp) drops dramatically. After removal of the adapters sequences, quality is really high, more than at the start of the reads, this is normal?? I mean i expect data to be better, but not at this level of improvement





truseq removal illumina adapters • 1.3k views
ADD COMMENTlink modified 3.3 years ago by harold.smith.tarheel4.3k • written 3.3 years ago by cristianrohr76830
gravatar for harold.smith.tarheel
3.3 years ago by
United States
harold.smith.tarheel4.3k wrote:

Yes, because the low quality in later cycles is largely the result of sequencing past the adapter ends. By trimming adapters (and subsequent sequences) you remove those low-quality data.

And read quality is always lower for the first ~five cycles of sequencing; search this forum or Seqanswers for an explanation.

ADD COMMENTlink written 3.3 years ago by harold.smith.tarheel4.3k
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 735 users visited in the last hour