RNA-Seq data validation by qPCR

Question

[kaczor.karolina]

Hi,

How could I validate the results of differentially expressed RNA by the use of RT-qPCR. What positive control should I use- is universal RNA good as a control for salivary RNA? Are other methods for validation of differentially expressed RNA? How to interpret the results of qPCR?

Thanks in advance

Answer 1

[Devon Ryan]

I've generally just ordered TaqMan primers, that's about the simplest method. I've never bothered with positive controls, they're not particularly useful. As for what to compare against, I can't give you any advice there, since I've never used salivary RNA.

[kaczor.karolina]

Thanks Ryan for your answer!