Biopython's GenomeDiagram input files???
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6.2 years ago
ab.tsubaki ▴ 50

Hi all

 

I am currently trying to draw up a synteny map using Biopython's GenomeDiagram package. I have been able to complete some examples with .gb files and now I want to try it on my own data.

Problem: my data is in fasta format????

Any one with experience on this?

1) Can GenomeDiagram use fasta files?

2) has anyone tried to convert fasta to genbank files?

 

Help will be much appreciated!

Regards

GenomeDiagram synteny biopython • 1.8k views
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can you post the code you've used with your genbank file?

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GenomeDiagram doesn't draw sequences - they are just the horizontal scale/axis, what you draw is usually annotation/features along the sequence (as boxes, arrows, etc). You're probably using the annotation from a GenBank file (e.g. genes as SeqFeature objects), but do you have similar annotation for your FASTA sequences?

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6.2 years ago
mgalactus ▴ 760

Hi,

Sure, you can use fasta files to draw genome diagrams; you could provide the features you want to draw on top of the sequence from other files (say a gff file or a simple table you might have). You can convert a fasta file to a Genbank file with the following python script:

from Bio import SeqIO

SeqIO.write(SeqIO.parse('input.fasta', 'fasta'), 'output.gbk', 'genbank')

Marco

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