**440**wrote:

Hello,

So I try to filter the DE genes and non-DE genes. What I'm confused is the value of p-value. As far as I understand, smaller p-value indicates that the data is not a coincidence. In this case, the difference on expression level between two groups is "really" different and not coincidence. In that case, usually the logFC will also have a quite big number (>1 or <-1).

If I tried to filter near 0 logFC (which is non-DE genes), the p-value is quite big and I assume the meaning is it can be "coincidence". Does it mean that the non-DE genes is actually "coincidence" or the p-value refer to the expression level set and the big p-value meaning is the data is not statistically difference and resulted with near 0 logFC? Is it enough to filter the non-DE genes by near 0 logFC or I also need to filter both near 0 logFC and small p-value (less than 0.05 maybe)?

Thank you in advance.

**10k**• written 4.1 years ago by bharata1803 •

**440**