Question: Discrepancy with MACS2.0 and MACS2.1 broad peaks
1
gravatar for ivivek_ngs
3.3 years ago by
ivivek_ngs4.7k
Seattle,WA, USA
ivivek_ngs4.7k wrote:

I have been trying to understand the difference between broad peaks called by macs2 and macs2.1 . I used the same parameters to call the broad peaks and I found there is a huge difference between the number of peaks that is being called. My parameters are p-value 1e-5 and broad-cutoff 0.1. I used this paramter to run on the same samples with MACS2 and MACS2.1. However I see a drastic jump in the number of peaks called with MACS2.1. Has anyone seen such a thing before? Total number of peaks in MACS2.1 is 4 times that of the number of peaks called by MACS2.0. I will try to see the overlap between the two peak files but is this a likely thing observed usually? Thanks
 

macs2.1 chip-seq macs2 • 973 views
ADD COMMENTlink written 3.3 years ago by ivivek_ngs4.7k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1187 users visited in the last hour