Discrepancy with MACS2.0 and MACS2.1 broad peaks
0
1
Entering edit mode
8.4 years ago
ivivek_ngs ★ 5.2k

I have been trying to understand the difference between broad peaks called by macs2 and macs2.1 . I used the same parameters to call the broad peaks and I found there is a huge difference between the number of peaks that is being called. My parameters are p-value 1e-5 and broad-cutoff 0.1. I used this parameter to run on the same samples with MACS2 and MACS2.1. However I see a drastic jump in the number of peaks called with MACS2.1. Has anyone seen such a thing before? Total number of peaks in MACS2.1 is 4 times that of the number of peaks called by MACS2.0. I will try to see the overlap between the two peak files but is this a likely thing observed usually? Thanks

ChIP-Seq MACS2.1 MACS2 • 2.0k views
ADD COMMENT

Login before adding your answer.

Traffic: 2922 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6