Question: What factors impact the total number reads produced by different sequencing instruments (e.g. HiSeq vs MiSeq)?
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gravatar for toddknutson
4.1 years ago by
toddknutson50
United States
toddknutson50 wrote:

I understand that different Illumina sequencers can output different numbers of reads (i.e. HiSeq instruments can produce  40-400 million reads/lane, whereas MiSeq can produce 5-25 million reads/lane). My question is why? What factors in the Illumina next-generation sequencing technology impact read count outputs from these sequencers? For example, can certain instrument settings increase or decrease the number of reads produced (assuming that equal amounts of DNA were supplied to the sequencer)?

 

sequencing next-gen • 1.7k views
ADD COMMENTlink modified 4.1 years ago by harold.smith.tarheel4.5k • written 4.1 years ago by toddknutson50
3
gravatar for harold.smith.tarheel
4.1 years ago by
United States
harold.smith.tarheel4.5k wrote:

The two factors that limit the amount of data are 1) the maximum cluster density (i.e., number of clones) that can be imaged and resolved by the camera, and 2) the surface area of the flow cell lane that's imaged (MiSeq flow cells are much smaller than HiSeq).

ADD COMMENTlink written 4.1 years ago by harold.smith.tarheel4.5k
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