Interpreting dispersion plots in RNAseq analysis
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6.8 years ago

I have a RNAseq experiment I'm analyzing, using the Tuxedo pipeline. One of the suggested plots in cummRbund is a dispersion plot that plots dispersion against mean counts. I understand what the plot shows, however I'm not sure about the interpretation. I have an example from cummRbund below, 3 treatments with 5 replicates each.

How do I decide if the dispersion is higher than it should be, are there any comparison values I can look at? Or is this mostly done to compare between sample groups rather than looking at the absolute values?

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RNA-Seq dispersion • 3.5k views
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