This is more of a wet lab related question but since it have might affected one or another of you I'll try my luck.
Does anyone of you have a reference or an idea of how efficient the library preparation (I know it might differ from protocol to protocol significantly) is? Some good estimates should give me a starting point.
Edit: To be more specific. What I mean is that from a fixed amount of DNA I can calculate how many molecules are in my sample. However, what I need to know is:
- How many of those molecules actually end up getting caught by the sample preparation?
- If this is possible to know: How much of the provided library is getting a place on the flowcell itself? (This might be the trickiest one since you can adjust with molarity of the library itself but for sake of simplicity assume there should be enough space for the whole library/ every molecule)?
Thanks in advance