Entering edit mode
8.3 years ago
michelle
▴
10
Hey All,
I obtained my PhastCons scores from USCS browser and sum scores for individual sites together. I was wondering if I average the sums by length, in what range (or a cutoff) it means my DNA element is highly conserved.
Thank you :)
Define highly.
Maybe look at a gene you consider highly conserved and measure it by the same method.
It is an enhancer element I found in mice. I want to see whether it is conserved across species. Should I use the average score? If so, like what is the cutoff for conservation.
PhastCons scores range between 0-1. So the score for any nucleotide varies from 0-1. You can sum the score for the length of your region and divide by the length to get the score between 0-1.
I did! But I was just wondering whether there is a cut off for conservation. How do I interpret my average score? In what range, it means it is definitely not conserved.