How to get Cufflinks data for MISO alternative splicing events?
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6.3 years ago

Hello,

i am currently doing an analysis on splice junctions at alternative exons, that belong to exon-skipping (ES) events. Therefore i have done the following steps so far:

  1. I downloaded mRNA-Seq reads from human spleen cells from GEO
  2. I used TopHat to mao these reads to hg19
  3. I used TopHats accepted_hits.bam file and an Ensemble gene annotation provided by the MISO homepage for transcript assembly and abundance estimation with Cufflinks
  4. I used TopHats accepted_hits.bam file and an ES event annotation from the MISO homepage for detection and quantification of ES events with MISO

I now have the following data:

  • assmebled_transcripts.gtf file from Cufflinks containing for each transcript an ensemble gene ID, exons, FPKM and some other values
  • misoresults.miso_summary file from MISO containing the ES events given as exon triplets and some properties for each ES event
  • ES_to_ensGene_hg19.txt file containing Ensemble gene ID(s) for each ES event in the MISO summary file above

Now, for each ES event in the MISO summary file i need to have the FPKM, TSS and TTS of the respective transcript. Which tool(s) can i use for that task? I hope that somebody can help me with that.

Best regards

MISO Cufflinks Ensembl annotation TSS FPKM • 2.5k views
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