Off topic:Concentrating method for TruSeq lib prep in microRNA sequencing
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5.5 years ago
CandiceChuDVM ★ 2.2k

Hi all,

We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).

We use column-based isolation methods and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples. 

I was wondering how can we concentrate our microRNA samples? 

Any suggestions are appreciated. Thanks!

myposts RNA-Seq microRNA TruSeq • 1.1k views
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