Question: (Closed) Concentrating method for TruSeq lib prep in microRNA sequencing
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gravatar for CandiceChuDVM
4.9 years ago by
CandiceChuDVM2.1k
United States/College Station/Texas A&M University
CandiceChuDVM2.1k wrote:

Hi all,

We are planning to submit our microRNA samples that we isolated from biofluids (serum and urine).

We use column-based isolation methods and we usually elude our samples in 50ul of RNase-free water; however, the requirement for sample submission (10-15 ng of microRNA in 5 ul) is way concentrated than our samples. 

I was wondering how can we concentrate our microRNA samples? 

Any suggestions are appreciated. Thanks!

rna-seq truseq microrna myposts • 1.0k views
ADD COMMENTlink written 4.9 years ago by CandiceChuDVM2.1k

Hello candy9087!

We believe that this post does not fit the main topic of this site.

This doesn't seem to be a bioinformatics question, better check seqanswers.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 4.9 years ago by Michael Dondrup48k
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