Question: Backround correction for an Agilent Data set
0
gravatar for shawin
3.2 years ago by
shawin10
United Kingdom
shawin10 wrote:

I used limma to read and normalize An Agilent data set , i did not apply the backround correction on it : which is :

y <- backgroundCorrect(x,method="normexp")# 

then i found another method which is nec(), neqc ()  :

y=nec(x, status=NULL, negctrl="negative", regular="regular", offset=16,robust=FALSE, detection.p="Detection")

but i received   an error :

Error in normexp.fit.detection.p(x, detection.p) : 
  Detection p values not found in the data.

please any suggestion 

 

 

 

R • 898 views
ADD COMMENTlink written 3.2 years ago by shawin10

You are going to need to give us more information, but the error implies that there are not detection p-values in the data.  

ADD REPLYlink written 3.2 years ago by Sean Davis25k
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