Question: Does mirna-seq is stranded?
0
gravatar for biobiu
3.9 years ago by
biobiu110
United States
biobiu110 wrote:

I'm using miRNA-seq data retrieved from the TCGA project.

When looking on the aligned sequences it seems that all sequences for the same small RNA molecule are on the same strand (in contrast to most RNA-seq that the sequences derived from both strands due to PCR amplification), Am I right, does the data indeed stranded?

 

sequencing rna-seq • 1.2k views
ADD COMMENTlink modified 3.9 years ago • written 3.9 years ago by biobiu110
1
gravatar for Chris Fields
3.9 years ago by
Chris Fields2.1k
University of Illinois Urbana-Champaign
Chris Fields2.1k wrote:

See this prior question for the answer.

Just a note, most modern RNA-Seq data tend to be strand-specific (at least they are for our group).  Cost of generating strand-specific libraries is pretty equivalent to non-strand-specific ones.  

 

 

ADD COMMENTlink written 3.9 years ago by Chris Fields2.1k
0
gravatar for biobiu
3.9 years ago by
biobiu110
United States
biobiu110 wrote:

Thanks for your comment! I believe that is the case for the mirna-seq I'm using.

 

ADD COMMENTlink written 3.9 years ago by biobiu110
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