Tool for simulating stranded RNA-Seq data

Question

[tsai.sw]

Hi there,

Has anyone came across any software that can simulate stranded RNA-Seq with FASTQ output?

[dariober]

Google'ing "RNA-Seq simulation" returns some hits, including this paper Polyester: simulating RNA-seq datasets with differential transcript expression

[tsai.sw]

Thanks, Polyester doesn't simulated "stranded" RNA-Seq, thanks for looking tho.

Answer 1

[Thibault D.]

Hi,

Here are two of them : Polyester and FluxSimulator. They have different kind of parameters, Flux Simulator is "mathematics-oriented". Polyester is IMHO easier to use.