Question: SMRT Analysis General Questions
4.9 years ago by
me • 10
me • 10 wrote:
A couple of questions I'm unsure about and can't get a definite answer for (yes, I've looked over the documentation, wikis, etc..):
- SMRT Analysis software installed on local server is self contained, correct? i.e.) does not connect/transfer any data to outside servers like PacBios or others.
- Do reference and de novo assemblies through the SMRT Portal yield structural variants reports? It doesn't make sense to me that I need to run another job to get variant calls (RS_Minor_Variant)...maybe I'm missing something here.
Note:I also see a 'Corrections' menu, showing INDELS...but are these "corrected", changed, on the final assembly sequence?
- Where's/how do I get the final assembled sequence?
Overall, how do I get a de novo assembly with variant call information; what's the correct protocol(s) to follow?
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