Question: Finding miRNA motifs from Exome sequencing
gravatar for bharata1803
4.3 years ago by
bharata1803450 wrote:


I got a suggestion from my supervisor to check cancer data and miRNA effect. All I can get from NCBI GEO is 7 pairs of RNA-seq data of normal-tumor cell and 8 pairs of whole exome seq data also from normal-tumor cell.

After reading several source and try 1 of the motif finding of miRNA in UTR, I have an idea for checking the relation of miRNA and gene expression. My idea is like this:

Check the miRNA binding motifs from the sample's 3'UTR. From this, I will get the possible miRNA which bind with mRNA for specific sample in a specific gene. Then, I will combine the gene expression data with the predicted miRNA which bind to those mRNA of those genes.

Is it possible to do that? I will need to get 3'UTR sequence from every samples which I think I can get from whole exome seq. Is it possible to get the 3'UTR from whole exome seq?

mirna exome-seq sequence • 1.4k views
ADD COMMENTlink written 4.3 years ago by bharata1803450

Any update on this? I also wonder if exome sequencing contains 3' UTR regions

ADD REPLYlink written 21 months ago by -_-850

the answer is no, exome seq only sequence known/targeted exon region

ADD REPLYlink written 20 months ago by bharata1803450
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1411 users visited in the last hour