Hi,
I've run a PCR, with the DNA phusion KIT, to get a fragment of 125 bp, from FFPE DNA.
As I analyzed the results using our Fragment Analyzer, I noticed a very weak band in the NT control of the same size as the Positive Control, which of course led to the conclusion of contamination.
HOWEVER, in the lanes where I added template DNA, there is of course a strong band of the desired size, but in one lane where I added a very low concentration of DNA there is no band what so ever. A rough estimate is that the DNA concentration in PCR reaction with no band is 0.28 ng/µl.
I am a bit confused about this as I would have expected bands in ALL samples, due to the faint suspected contamination?
What could be the cause of this? Primer Dimers in purely empty samples?
