In our group we are about to design an amplicon-based targeted panel for NGS, and we're aiming at around 150 bp for mean amplicon sizes. The wet lab people came back to me asking to calculate the optimal number of samples to be loaded in the instrument (MiSeq). And this is where I have my question: ideally I'd like to pick 2x150bp as read length, so I can have a greater output, however the mean amplicon size might be close to the read length, or in some cases, lower.
Hence my question: would it be "safe" to use a read length approximately equal to the amplicon length so that it doesn't complicate my analysis job afterwards (I'm thinking about adapter read through)? My previous experience always had a larger amplicon length (> 200 bp) so it can't really compare.
Should I still go for 2x150bp, or aim lower at 2x75bp? If I use 2x150bp, would possible adapter read through throw a spanner in the works, or should soft clipping (from BWA) be enough?