Paper(S) Discussing Issues In Quality Assessment Of Ngs Data
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10.7 years ago
Paul ▴ 760

Hi,

I'm wondering if anyone is aware of any good review papers outlining the main issues in quality assessment of NGS data, particularly Illumina RNA-seq data if at all possible.

I've used FastQC for quality assessment, but it isn't published and I'm wondering if there are other papers which back it up.

Thanks!

next-gen sequencing rna quality • 2.2k views
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Entering edit mode
10.7 years ago

Take a look at the picard CollectRnaSeqMetrics tool. I'm not suggesting that it is the only tool, but it does give some useful statistics and will certainly highlight samples that are outliers after alignment.

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10.7 years ago
Ian 5.9k

You might find this BioStar link of use.

My own suggestion of htSeqTools and HOMER is still for general NGS datasets.

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