Encoding quality score for different NGS platform.
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5.5 years ago
mieszko91 ▴ 30

Hi! I am looking for information about encoding quality score for different NGS platforms.

Here are descibed encoding methods for Illumina platform (Phred33 and Phred64). https://en.wikipedia.org/wiki/FASTQ_format#Encoding

How quality score is encoded in fastq files from other platforms like SOLID, IonTorrent or Roche454?

next-gen Encoding • 1.8k views
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Q-scores on LifeTech instruments are in sanger fastq format but the actual scores are calculated differently than other platforms. Current supported values appear to be 0-44. You will need to create an account on the ioncommunity web site (they keep most information related to ion behind a login required wall) to view some of this information.

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